Molecular Fingerprinting of Porphyromonas Gingivalis by PCR of Repetitive Extragenic Palindromic (REP) Sequences and Comparison with other Fingerprinting Methods
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منابع مشابه
DNA Fingerprinting Based on Repetitive Sequences of Iranian Indigenous Lactobacilli Species by (GTG)5- REP-PCR
Background and Objective: The use of lactobacilli as probiotics requires the application of accurate and reliable methods for the detection and identification of bacteria at the strain level. Repetitive sequence-based polymerase chain reaction (rep-PCR), a DNA fingerprinting technique, has been successfully used as a powerful molecular typing method to determine taxonomic and phylogenetic relat...
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Pseudomonas putida KT2440 is a soil bacterium that effectively colonises the roots of many plants and degrades a variety of toxic aromatic compounds. Its genome has recently been sequenced. We describe that a 35 bp sequence with the structure of an imperfect palindrome, originally found repeated three times downstream of the rpoH gene terminator, is detected more than 800 times in the chromosom...
متن کاملClustering of South African Helicobacter pylori isolates from peptic ulcer disease patients is demonstrated by repetitive extragenic palindromic-PCR fingerprinting.
The present report assesses the association between clonal groupings, disease, and the virulence fingerprint of 76 South African Helicobacter pylori cagA(+) strains isolated from 57 Cape-colored subjects. Two methods, repetitive extragenic palindromic (REP)-PCR and random amplified polymorphic DNA (RAPD)-PCR, were used to generate DNA fingerprints, and computer-assisted analysis was used to der...
متن کاملCharacterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting.
Thirteen isolates of Nocardia asteroides from both soils and aquatic samples (lake and moat sediments, as well as scum from activated sludge), together with a type strain and two known clinical isolates of this species, were characterized by repetitive extragenic palindromic-PCR fingerprinting with the BOX-A1R primer. The resulting DNA fingerprint patterns proved to be strain specific, and clus...
متن کاملmolecular analysis of pseudomonas aeruginosa strains isolated from burn patients by repetitive extragenic palindromic-pcr (rep-pcr)
conclusions according to the findings of our study, there were diverse and high-level resistant p. aeruginosa strains in the major burn center in khuzestan. therefore, we faced troubles controlling the diverse p. aeruginosa clones in the burn patients. methods in a cross sectional study, a total of 75 strains of p. aeruginosa were isolated from burn patients at taleghani hospital, which is the ...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 1999
ISSN: 0022-2615,1473-5644
DOI: 10.1099/00222615-48-8-741